High-throughput sequencing identification of novel and conserved miRNAs in the Brassica oleracea leaves.

ABSTRACT:

Background

Plant microRNAs are short (~21 nt) non-coding molecules that regulate gene expression by targeting the mRNA cleavage or protein translation inhibition. In this manner, they play many important roles in the cells of living organisms. One of the plant species in which the entire set of miRNAs has not been yet completely identified is Brassica oleracea var. capitata (cabbage). For this reason and for the economic and nutritional importance of this food crop, high-throughput small RNAs sequencing has been performed to discover the novel and conserved miRNAs in mature cabbage leaves.

Results

In this study, raw reads generated from three small RNA libraries were bioinformatically processed and further analyzed to select sequences homologous to known B. oleracea and othe

SUBMITTER: Lukasik A 

PROVIDER: S-EPMC3840582 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature