Annexin A7 suppresses lymph node metastasis of hepatocarcinoma cells in a mouse model.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer death in China. This study investigated the effects of Annexin A7 (ANXA7) on the inhibition of HCC lymph node metastasis in a mouse model.

Methods

The stable knockup and knockdown of Annexin A7-expressing HCC cells using Annexin A7 cDNA and shRNA vectors, respectively, were injected into a mouse footpad to establish primary and metastatic tumors in mice. On the 14th, 21st, and 28th days after HCC cells inoculation, the mice were sacrificed for inspection of primary and secondary tumors and immunohistochemistry of Annexin A7 expression.

Results

The lymph node metastasis rate of the FANXA7-control group was 77%, and the lymph node metastasis rate of the FANXA7-down group was 100% (p<0.05).

SUBMITTER: Jin Y 

PROVIDER: S-EPMC3840638 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature