Intra-specific variability and biological relevance of P3N-PIPO protein length in potyviruses.

ABSTRACT:

Background

Pipo was recently described as a new ORF encoded within the genome of the Potyviridae family members (PNAS 105:5897-5902, 2008). It is embedded within the P3 cistron and is translated in the +2 reading frame relative to the potyviral long ORF as the P3N-PIPO fusion protein. In this work, we first collected pipo nucleotide sequences available for different isolates of 48 Potyvirus species. Second, to determine the biological implications of variation in pipo length, we measured infectivity, viral accumulation, cell-to-cell and systemic movements for two Turnip mosaic virus (TuMV) variants with pipo alleles of different length in three different susceptible host species, and tested for differences between the two variants.

Results

In addition to inter-specific varia

SUBMITTER: Hillung J 

PROVIDER: S-EPMC3840659 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature