Computational insights into the interactions between DNA and siRNA with "rigid" and "flexible" triazine dendrimers.

ABSTRACT: In this study, simulation challenges intuitive models of "flexible" and "rigid" generation two triazine dendrimers as it pertains to solution conformation and conformation on binding DNA or siRNA sequences. These results derive from structural and energetic analyses of the binding events. Simulations of the rigid structure reinforce the role of the constrained piperazine linker in positioning the peripheral groups at significant distance from each other and the core of the dendrimer. In contrast, the flexible dendrimer, characterized by triethyleneglycol-like linkers, collapses in solution. On binding DNA and siRNA, these conformations are largely retained. The rigid dendrimer undergoes reorganization of peripheral groups to generate a large number of contacts to the nucleic acid. In contr

SUBMITTER: Pavan GM 

PROVIDER: S-EPMC3841066 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature