Transcription factor Kruppel-like factor 2 plays a vital role in endothelial colony forming cells differentiation.

ABSTRACT:

Aims

Endothelial colony forming cells (ECFCs) participate in post-natal vasculogenesis. We previously reported that vascular endothelial growth factor (VEGF) promotes human ECFC differentiation through AMP-activated protein kinase (AMPK) activation. However, the mechanisms underlying transcriptional regulation of ECFC differentiation still remain largely elusive. Here, we investigated the role of transcription factor Krüppel-like factor 2 (KLF2) in the regulation of ECFC function.

Methods and results

Human ECFCs were isolated from cord blood and cultured. Treatment with VEGF significantly increased endothelial markers in ECFCs and their capacity for migration and tube formation. The mRNA and protein levels of KLF2 were also significantly up-regulated. This up-regulation was

SUBMITTER: Song Y 

PROVIDER: S-EPMC3841418 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature