EP1: a novel rabbit monoclonal antibody for detection of oestrogen receptor α.

ABSTRACT:

Aims

Assessment of hormone receptor expression is part of routine examination of every breast cancer. In this study, we report the characterisation of a novel rabbit monoclonal antibody, clone EP1, directed against oestrogen receptor (ER) α. Additionally, its immunohistochemical performance characteristics in archival tissues are evaluated in normal tissues and two distinct cohorts of breast cancer patients.

Methods

Comparative analyses between EP1 and the anti-ERα component of the ER/PR pharmDx kit (cocktail of mouse monoclonal antibody clones 1D5 and ER-2-123) and between EP1 and another commercially available rabbit monoclonal antibody, clone SP1, are described.

Results

Clone EP1 specifically detects nuclear ER in all tissues examined; cytoplasmic staining was not

SUBMITTER: Badve S 

PROVIDER: S-EPMC3841770 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature