Methods to detect replication-dependent and replication-independent DNA structure-induced genetic instability.

ABSTRACT: DNA can adopt a variety of alternative secondary (i.e., non-B DNA) conformations that play important roles in cellular metabolism, including genetic instability, disease etiology and evolution. While we still have much to learn, research in this field has expanded dramatically in the past decade. We have summarized in our previous Methods review (Wang et al., Methods, 2009) some commonly used techniques to determine non-B DNA structural conformations and non-B DNA-induced genetic instability in prokaryotes and eukaryotes. Since that time, we and others have further characterized mechanisms involved in DNA structure-induced mutagenesis and have proposed both replication-dependent and replication-independent models. Thus, in this review, we highlight some current methodologies to identify DN

SUBMITTER: Wang G 

PROVIDER: S-EPMC3842213 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature