Proteomic and genomic analyses of antimony resistant Leishmania infantum mutant.

ABSTRACT:

Background

Antimonials remain the primary antileishmanial drugs in most developing countries. However, drug resistance to these compounds is increasing and our understanding of resistance mechanisms is partial.

Methods/principal findings

In the present study, quantitative proteomics using stable isotope labelling of amino acids in cell culture (SILAC) and genome next generation sequencing were used in order to better characterize in vitro generated Leishmania infantum antimony resistant mutant (Sb2000.1). Using the proteomic method, 58 proteins were found to be differentially regulated in Sb2000.1. The ABC transporter MRPA (ABCC3), a known marker of antimony resistance, was observed for the first time in a proteomic screen. Furthermore, transfection of its gene conferred ant

SUBMITTER: Brotherton MC 

PROVIDER: S-EPMC3842243 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature