Testing the reproducibility of multiple displacement amplification on genomes of clonal endosymbiont populations.

ABSTRACT: The multiple displacement amplification method has revolutionized genomic studies of uncultured bacteria, where the extraction of pure DNA in sufficient quantity for next-generation sequencing is challenging. However, the method is problematic in that it amplifies the target DNA unevenly, induces the formation of chimeric reads and also amplifies contaminating DNA. Here, we have tested the reproducibility of the multiple displacement amplification method using serial dilutions of extracted genomic DNA and intact cells from the cultured endosymbiont Bartonella australis. The amplified DNA was sequenced with the Illumina sequencing technology, and the results were compared to sequence data obtained from unamplified DNA in this study as well as from a previously published genome project. We s

SUBMITTER: Ellegaard KM 

PROVIDER: S-EPMC3842359 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature