Investigating xylose metabolism in recombinant Saccharomyces cerevisiae via 13C metabolic flux analysis.

ABSTRACT:

Background

To engineer Saccharomyces cerevisiae for efficient xylose utilization, a fungal pathway consisting of xylose reductase, xylitol dehydrogenase, and xylulose kinase is often introduced to the host strain. Despite extensive in vitro studies on the xylose pathway, the intracellular metabolism rewiring in response to the heterologous xylose pathway remains largely unknown. In this study, we applied 13C metabolic flux analysis and stoichiometric modeling to systemically investigate the flux distributions in a series of xylose utilizing S. cerevisiae strains.

Results

As revealed by 13C metabolic flux analysis, the oxidative pentose phosphate pathway was actively used for producing NADPH required by the fungal xylose pathway during xylose utilization of recombinant S. cer

SUBMITTER: Feng X 

PROVIDER: S-EPMC3842631 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature