Large-scale analysis of peptide sequence variants: the case for high-field asymmetric waveform ion mobility spectrometry.

ABSTRACT: Large scale analysis of proteins by mass spectrometry is becoming increasingly routine; however, the presence of peptide isomers remains a significant challenge for both identification and quantitation in proteomics. Classes of isomers include sequence inversions, structural isomers, and localization variants. In many cases, liquid chromatography is inadequate for separation of peptide isomers. The resulting tandem mass spectra are composite, containing fragments from multiple precursor ions. The benefits of high-field asymmetric waveform ion mobility spectrometry (FAIMS) for proteomics have been demonstrated by a number of groups, but previously work has focused on extending proteome coverage generally. Here, we present a systematic study of the benefits of FAIMS for a key challenge in pr

SUBMITTER: Creese AJ 

PROVIDER: S-EPMC3842852 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature