Caloric restriction-mediated induction of lipid metabolism gene expression in liver is enhanced by Keap1-knockdown.

ABSTRACT:

Purpose

CR increases fatty acid oxidation to decrease tissue lipid content. The Nuclear factor E2-related factor 2 (Nrf2)-Kelch like ECH associated Protein 1 (Keap1) pathway is an antioxidant gene regulatory pathway that has been previously investigated in weight gain. However, limited interaction of Nrf2/Keap1 and CR exists. The purpose of this study was to determine how Keap1 knockdown (Keap1-KD), which is known to increase Nrf2 activity, affects the CR response, such as weight loss, hepatic lipid decrease, and induction of fatty acid oxidation gene expression.

Methods

C57BL/6 and Keap1-KD mice were maintained on 40% CR or fed ad libitum for 6 weeks. Hepatic lipid content, lipid metabolic gene, and miRNA expression was quantified.

Results

CR lowered hepatic lipid co

SUBMITTER: Kulkarni SR 

PROVIDER: S-EPMC3843954 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature