Correlation between centromere protein-F autoantibodies and cancer analyzed by enzyme-linked immunosorbent assay.

ABSTRACT:

Background

Centromere protein-F (CENP-F) is a large nuclear protein of 367 kDa, which is involved in multiple mitosis-related events such as proper assembly of the kinetochores, stabilization of heterochromatin, chromosome alignment and mitotic checkpoint signaling. Several studies have shown a correlation between CENP-F and cancer, e.g. the expression of CENP-F has been described to be upregulated in cancer cells. Furthermore, several studies have described a significant correlation between the expression of autoantibodies to CENP-F and cancer.

Methods

Autoantibodies to CENP-F were detected in a small number of samples during routine indirect immunofluorescence (IIF) analysis for anti-nuclear antibodies (ANA) using HEp-2 cells as substrate. Using overlapping synthetic pepti

SUBMITTER: Welner S 

PROVIDER: S-EPMC3844405 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature