Whole transcriptome sequencing identifies tumor-specific mutations in human oral squamous cell carcinoma.

ABSTRACT:

Background

The accumulation of somatic mutations in genes and molecular pathways is a major factor in the evolution of oral squamous cell carcinoma (OSCC), which sparks studies to identify somatic mutations with clinical potentials. Recently, massively parallel sequencing technique has started to revolutionize biomedical studies, due to the rapid increase in its throughput and drop in cost. Hence sequencing of whole transcriptome (RNA-Seq) becomes a superior approach in cancer studies, which enables the detection of somatic mutations and accurate measurement of gene expression simultaneously.

Methods

We used RNA-Seq data from tumor and matched normal samples to investigate somatic mutation spectrum in OSCC.

Results

By applying a sophisticated bioinformatic pipeline, w

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC3844419 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature