Targeting focal adhesion turnover in invasive breast cancer cells by the purine derivative reversine.

ABSTRACT:

Background

The dynamics of focal adhesion (FA) turnover is a key determinant for the regulation of cancer cell migration. Here we investigated FA turnover in a panel of breast cancer models with distinct invasive properties and evaluated the impact of reversine on this turnover in relation to cancer cell invasion in in vitro and in vivo conditions.

Methods

Live imaging and immunofluorescence assays were used to investigate FA turnover in breast cancer cells. Biochemical studies were used to investigate the impact of reversine on FA signalling and turnover. In vivo activity was investigated using orthotopic breast cancer mouse models.

Results

Accelerated FA disassembly from plasma membrane protrusions was observed in invasive compared with non-invasive breast cancer ce

SUBMITTER: Bijian K 

PROVIDER: S-EPMC3844920 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature