Evaluating the fitness cost of protein expression in Saccharomyces cerevisiae.

ABSTRACT: Protein metabolism is one of the most costly processes in the cell and is therefore expected to be under the effective control of natural selection. We stimulated yeast strains to overexpress each single gene product to approximately 1% of the total protein content. Consistent with previous reports, we found that excessive expression of proteins containing disordered or membrane-protruding regions resulted in an especially high fitness cost. We estimated these costs to be nearly twice as high as for other proteins. There was a ten-fold difference in cost if, instead of entire proteins, only the disordered or membrane-embedded regions were compared with other segments. Although the cost of processing bulk protein was measurable, it could not be explained by several tested protein features,

SUBMITTER: Tomala K 

PROVIDER: S-EPMC3845635 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature