S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis.

ABSTRACT:

Purpose

The receptor for advanced glycation end products (RAGE) contributes to multiple pathologies, including diabetes, arthritis, neurodegenerative diseases, and cancer. Despite the obvious need, no RAGE inhibitors are in common clinical use. Therefore, we developed a novel small RAGE antagonist peptide (RAP) that blocks activation by multiple ligands.

Experimental design

RAGE and its ligands were visualized by immunohistochemical analysis of human pancreatic tissues, and siRNA was used to analyze their functions. Interactions between RAGE and S100P, S100A4, and HMGB-1 were measured by ELISA. Three S100P-derived small antagonistic peptides were designed, synthesized, and tested for inhibition of RAGE binding. The effects of the peptide blockers on NFκB-luciferase reporter

SUBMITTER: Arumugam T 

PROVIDER: S-EPMC3845828 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature