Screening of a Brassica napus bacterial artificial chromosome library using highly parallel single nucleotide polymorphism assays.

ABSTRACT:

Background

Efficient screening of bacterial artificial chromosome (BAC) libraries with polymerase chain reaction (PCR)-based markers is feasible provided that a multidimensional pooling strategy is implemented. Single nucleotide polymorphisms (SNPs) can be screened in multiplexed format, therefore this marker type lends itself particularly well for medium- to high-throughput applications. Combining the power of multiplex-PCR assays with a multidimensional pooling system may prove to be especially challenging in a polyploid genome. In polyploid genomes two classes of SNPs need to be distinguished, polymorphisms between accessions (intragenomic SNPs) and those differentiating between homoeologous genomes (intergenomic SNPs). We have assessed whether the highly parallel Illumina Golde

SUBMITTER: Cao HX 

PROVIDER: S-EPMC3846124 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature