Detection of dengue viruses using reverse transcription-loop-mediated isothermal amplification.

ABSTRACT:

Background

Early and rapid detection of dengue virus (DENV) infection during the febrile period is crucial for proper patient management and prevention of disease spread. An easy to perform and highly sensitive method is needed for routine implementation especially in the resource-limited rural healthcare settings where dengue is endemic.

Methods

A single-tube reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay with a set of nine primers was developed for the detection of all four DENV serotypes and their different genotypes. The sensitivity and specificity of the RT-LAMP were evaluated. The clinical applicability of RT-LAMP assay for detection of DENV RNA was assessed in a total of 305 sera of clinically-suspected dengue patients. The test results o

SUBMITTER: Teoh BT 

PROVIDER: S-EPMC3846474 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature