Techniques for processing eyes implanted with a retinal prosthesis for localized histopathological analysis.

ABSTRACT: With the recent development of retinal prostheses, it is important to develop reliable techniques for assessing the safety of these devices in preclinical studies. However, the standard fixation, preparation, and automated histology procedures are not ideal. Here we describe new procedures for evaluating the health of the retina directly adjacent to an implant. Retinal prostheses feature electrode arrays in contact with eye tissue. Previous methods have not been able to spatially localize the ocular tissue adjacent to individual electrodes within the array. In addition, standard histological processing often results in gross artifactual detachment of the retinal layers when assessing implanted eyes. Consequently, it has been difficult to assess localized damage, if present, caused by impla

SUBMITTER: Nayagam DA 

PROVIDER: S-EPMC3846726 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature