Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans.

ABSTRACT:

Background

Building reference libraries of DNA barcodes is relatively straightforward when specifically designed primers are available to amplify the COI-5P region from a relatively narrow taxonomic group (e.g. single class or single order). DNA barcoding marine communities have been comparatively harder to accomplish due to the broad taxonomic diversity and lack of consistently efficient primers. Although some of the so-called "universal" primers have been relatively successful, they still fail to amplify COI-5P of many marine animal groups, while displaying random success even among species within each group. Here we propose a new pair of primers designed to enhance amplification of the COI-5P region in a wide range of marine organisms.

Results

Amplification tests conducte

SUBMITTER: Lobo J 

PROVIDER: S-EPMC3846737 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature