High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes.

ABSTRACT:

Background

High resolution melting analysis (HRM) is a rapid and cost-effective technique for the characterisation of PCR amplicons. Because the reverse genetics of segmented influenza A viruses allows the generation of numerous influenza A virus reassortants within a short time, methods for the rapid selection of the correct recombinants are very useful.

Methods

PCR primer pairs covering the single nucleotide polymorphism (SNP) positions of two different influenza A H5N1 strains were designed. Reassortants of the two different H5N1 isolates were used as a model to prove the suitability of HRM for the selection of the correct recombinants. Furthermore, two different cycler instruments were compared.

Results

Both cycler instruments generated comparable average melting

SUBMITTER: Kalthoff D 

PROVIDER: S-EPMC3847122 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature