Engineering E. coli strain for conversion of short chain fatty acids to bioalcohols.

ABSTRACT:

Background

Recent progress in production of various biofuel precursors and molecules, such as fatty acids, alcohols and alka(e)nes, is a significant step forward for replacing the fossil fuels with renewable fuels. A two-step process, where fatty acids from sugars are produced in the first step and then converted to corresponding biofuel molecules in the second step, seems more viable and attractive at this stage. We have engineered an Escherichia coli strain to take care of the second step for converting short chain fatty acids into corresponding alcohols by using butyrate kinase (Buk), phosphotransbutyrylase (Ptb) and aldehyde/alcohol dehydrogenase (AdhE2) from Clostridium acetobutylicum.

Results

The engineered E. coli was able to convert butyric acid and other short chain

SUBMITTER: Mattam AJ 

PROVIDER: S-EPMC3847231 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature