Towards practical time-of-flight secondary ion mass spectrometry lignocellulolytic enzyme assays.

ABSTRACT:

Background

Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) is a surface sensitive mass spectrometry technique with potential strengths as a method for detecting enzymatic activity on solid materials. In particular, ToF-SIMS has been applied to detect the enzymatic degradation of woody lignocellulose. Proof-of-principle experiments previously demonstrated the detection of both lignin-degrading and cellulose-degrading enzymes on solvent-extracted hardwood and softwood. However, these preliminary experiments suffered from low sample throughput and were restricted to samples which had been solvent-extracted in order to minimize the potential for mass interferences between low molecular weight extractive compounds and polymeric lignocellulose components.

Results

The pre

SUBMITTER: Goacher RE 

PROVIDER: S-EPMC3847459 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature