Analysis of serum protein glycosylation by a differential lectin immunosorbant assay (dLISA).

ABSTRACT:

Background

Lectin immunosorbant assays (LISAs) have been widely used for analyzing protein glycosylation. However, the analysis of serum samples by LISAs could suffer from high sample-dependent background noise. The aim of this study is to develop a differential lectin immunosorbant assay (dLISA) with reduced background interferences.

Methods

For the analysis of protein glycosylation, dLISA establishes a dose-response curve for every serum sample. The sample is split into five aliquots. Four aliquots undergo differential removal of the glycoprotein of interest by immunoprecipitation. Then, all five aliquots are subject to two measurements: protein by immunoassay and protein glycans by LISA. A dose-response curve is established by plotting glycans signals on the y-axis and pr

SUBMITTER: Li D 

PROVIDER: S-EPMC3847486 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature