HeLa cell response proteome alterations induced by mammalian reovirus T3D infection.

ABSTRACT:

Background

Cells are exposed to multiple stressors that induce significant alterations in signaling pathways and in the cellular state. As obligate parasites, all viruses require host cell material and machinery for replication. Virus infection is a major stressor leading to numerous induced modifications. Previous gene array studies have measured infected cellular transcriptomes. More recently, mass spectrometry-based quantitative and comparative assays have been used to complement such studies by examining virus-induced alterations in the cellular proteome.

Methods

We used SILAC (stable isotope labeling with amino acids in cell culture), a non-biased quantitative proteomic labeling technique, combined with 2-D HPLC/mass spectrometry and reciprocal labeling to identify and

SUBMITTER: Coombs KM 

PROVIDER: S-EPMC3847587 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature