Combining next-generation sequencing and online databases for microsatellite development in non-model organisms.

ABSTRACT: Next-generation sequencing (NGS) is revolutionising marker development and the rapidly increasing amount of transcriptomes published across a wide variety of taxa is providing valuable sequence databases for the identification of genetic markers without the need to generate new sequences. Microsatellites are still the most important source of polymorphic markers in ecology and evolution. Motivated by our long-term interest in the adaptive radiation of a non-model species complex of whitefishes (Coregonus spp.), in this study, we focus on microsatellite characterisation and multiplex optimisation using transcriptome sequences generated by Illumina® and Roche-454, as well as online databases of Expressed Sequence Tags (EST) for the study of whitefish evolution and demographic history. We ide

SUBMITTER: Rico C 

PROVIDER: S-EPMC3847856 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature