Developmental exposure of fetal ovaries and fetal germ cells to endometriosis in an endometriosis model causes differential gene expression in the preimplantation embryos of the first-generation and second-generation embryos.

ABSTRACT:

Objective

To characterize multigenerational gene expression anomalies in eight-cell stage embryos associated with developmental exposure to endometriosis.

Design

Using an endometriosis model in rats (F0 founder generation) to evaluate gene expression in F1 (fetal exposure) and F2 (fetal germ cell exposure) generation eight-cell stage embryos.

Setting

Laboratory.

Animal(s)

Endometriosis model in rats (Endo) and controls (Sham).

Intervention(s)

F0 Endo and Sham rats were bred; half the pregnant rats were killed on gestational day 3 to collect F1 eight-cell stage embryos and the others gestated to term (F1 females). Adult F1 females bred; F2 eight-cell embryos collected.

Main outcome measure(s)

Maintenance of differential gene expression in F1 and F2

SUBMITTER: Birt JA 

PROVIDER: S-EPMC3847911 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature