Organization of Plasmodium falciparum spliceosomal core complex and role of arginine methylation in its assembly.

ABSTRACT:

Background

Splicing and alternate splicing are the two key biological processes that result in the generation of diverse transcript and protein isoforms in Plasmodium falciparum as well as in other eukaryotic organisms. Not much is known about the organization of splicing machinery and mechanisms in human malaria parasite. Present study reports the organization and assembly of Plasmodium spliceosome Sm core complex.

Methods

Presence of all the seven Plasmodium Sm-like proteins in the intra-erythrocytic stages was assessed based on the protein(s) expression analysis using immuno-localization and western blotting. Localization/co-localization studies were performed by immunofluorescence analysis on thin parasite smear using laser scanning confocal microscope. Interaction studi

SUBMITTER: Hossain M 

PROVIDER: S-EPMC3848767 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature