Engineering xylose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production.

ABSTRACT:

Background

There has been a great deal of interest in fuel productions from lignocellulosic biomass to minimize the conflict between food and fuel use. The bioconversion of xylose, which is the second most abundant sugar present after glucose in lignocellulosic biomass, is important for the development of cost effective bioprocesses to fuels. Rhodococcus opacus PD630, an oleaginous bacterium, accumulates large amounts of triacylglycerols (TAGs), which can be processed into advanced liquid fuels. However, R. opacus PD630 does not metabolize xylose.

Results

We generated DNA libraries from a Streptomyces bacterium capable of utilizing xylose and introduced them into R. opacus PD630. Xsp8, one of the engineered strains, was capable of growing on up to 180 g L-1 of xylose. Xsp8 g

SUBMITTER: Kurosawa K 

PROVIDER: S-EPMC3848844 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature