Probing the ArcA regulon under aerobic/ROS conditions in Salmonella enterica serovar Typhimurium.

ABSTRACT:

Background

Hydrogen peroxide (H₂O₂) is a reactive oxygen species (ROS), which is part of the oxidative burst encountered upon internalization of Salmonella enterica serovar Typhimurium (S. Typhimurium) by phagocytic cells. It has previously been established that, the ArcAB two-component system plays a critical role in ROS resistance, but the genes regulated by the system remained undetermined to date. We therefore investigated the ArcA regulon in aerobically growing S. Typhimurium before and after exposure to H₂O₂ by querying gene expression and other physiological changes in wild type and ΔarcA strains.

Results

In the ΔarcA strain, expression of 292 genes showed direct or indirect regulation by ArcA in response to H₂O₂, of which 141were also regulated in aerobiosis, but in

SUBMITTER: Morales EH 

PROVIDER: S-EPMC3848847 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature