The Nedd4-binding protein 3 (N4BP3) is crucial for axonal and dendritic branching in developing neurons.

ABSTRACT:

Background

Circuit formation in the nervous system essentially relies on the proper development of neurons and their processes. In this context, the ubiquitin ligase Nedd4 is a crucial modulator of axonal and dendritic branching.

Results

Herein we characterize the Nedd4-binding protein 3 (N4BP3), a Fezzin family member, during nerve cell development. In developing rat primary hippocampal neurons, endogenous N4BP3 localizes to neuronal processes, including axons and dendrites. Transient in vitro knockdown of N4BP3 in hippocampal cultures during neuritogenesis results in impaired branching of axons and dendrites. In line with these findings, in vivo knockdown of n4bp3 in Xenopus laevis embryos results in severe alteration of cranial nerve branching.

Conclusions

We intro

SUBMITTER: Schmeisser MJ 

PROVIDER: S-EPMC3849298 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature