Cross-talk between E. coli strains and a human colorectal adenocarcinoma-derived cell line.

ABSTRACT: Although there is great interest in the specific mechanisms of how gut microbiota modulate the biological processes of the human host, the extent of host-microbe interactions and the bacteria-specific metabolic activities for survival in the co-evolved gastrointestinal environment remain unclear. Here, we demonstrate a comprehensive comparison of the host epithelial response induced by either a pathogenic or commensal strain of Escherichia coli using a multi-omics approach. We show that Caco-2 cells incubated with E. coli display an activation of defense response genes associated with oxidative stress. Indeed, in the bacteria co-culture system, the host cells experience an altered environment compared with the germ-free system that includes reduced pH, depletion of major energy substrates,

SUBMITTER: He X 

PROVIDER: S-EPMC3849634 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature