Exogenous mRNA delivery and bioavailability in gene transfer mediated by piggyBac transposition.

ABSTRACT:

Background

Up to now, the different uptake pathways and the subsequent intracellular trafficking of plasmid DNA have been largely explored. By contrast, the mode of internalization and the intracellular routing of an exogenous mRNA in transfected cells are poorly investigated and remain to be elucidated. The bioavailability of internalized mRNA depends on its intracellular routing and its potential accumulation in dynamic sorting sites for storage: stress granules and processing bodies. This question is of particular significance when a secure transposon-based system able to integrate a therapeutic transgene into the genome is used. Transposon vectors usually require two components: a plasmid DNA, carrying the gene of interest, and a source of transposase allowing the integration o

SUBMITTER: Bire S 

PROVIDER: S-EPMC3849706 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature