Cloning and functional characterization of the proton-coupled electrogenic folate transporter and analysis of its expression in retinal cell types.

ABSTRACT:

Purpose

In a prior study the cellular uptake of folate was investigated in the retina. Recently, a new proton-coupled folate transporter (PCFT) in human intestine was reported. In the present study, the expression of this novel transporter in the retina was determined, the mouse orthologue was cloned from retinal tissue, and its transport function was characterized.

Methods

RT-PCR and folate uptake measurements were used to detect the expression of PCFT in mouse retina and in retinal cell types. The expression of PCFT mRNA in intact retina was investigated by in situ hybridization. Mouse PCFT cDNA was cloned, and its transport characteristics were analyzed by electrophysiological methods after expression of the cloned transporter in Xenopus laevis oocytes.

Results

RT-

SUBMITTER: Umapathy NS 

PROVIDER: S-EPMC3850295 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature