Surface display of recombinant proteins on Escherichia coli by BclA exosporium of Bacillus anthracis.

ABSTRACT:

Background

The anchoring motif is one of the most important aspects of cell surface display as well as efficient and stable display of target proteins. Thus, there is currently a need for the identification and isolation of novel anchoring motifs.

Results

A system for the display of recombinant proteins on the surface of Escherichia coli was developed using the Bacillus anthracis exosporal protein (BclA) as a new anchoring motif. For the surface display of recombinant proteins, the BAN display platform was constructed in which a target protein is linked to the C-terminus of N-terminal domain (21 amino acids) of BclA. The potential application of BAN platform for cell surface display was demonstrated with two model proteins of different size, the Bacillus sp. endoxylanase (Xy

SUBMITTER: Park TJ 

PROVIDER: S-EPMC3850424 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature