Human casein alpha s1 (CSN1S1) skews in vitro differentiation of monocytes towards macrophages.

ABSTRACT:

Background

The milk-derived protein human Casein alpha s1 (CSN1S1) has recently been detected in blood cells and was shown to possess proinflammatory properties. In the present study, we investigated the effect of CSN1S1 on the differentiation of monocytes.

Methods

Primary human monocytes were stimulated with recombinant CSN1S1 and compared to cells stimulated with GM-CSF/IL-4 or M-CSF/IFNγ. Morphological changes were assessed by microscopy and quantification of surface markers of differentiation by FACS analysis. Phagocytic activity of CSN1S1 stimulated cells was measured by quantification of zymosan labeled particle uptake. The role of mitogen activated protein kinases for CSN1S1-induced differentiation of monocytes and proinflammatory cytokine expression was assessed by s

SUBMITTER: Vordenbaumen S 

PROVIDER: S-EPMC3850642 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature