Performance of five molecular methods for monitoring Arcobacter spp.

ABSTRACT:

Background

Bacteria belonging to the Arcobacter genus are emerging enteropathogens and potential zoonotic agents. Their taxonomy has evolved very rapidly, and there are presently 18 recorded species. The prevalence of species belonging to Arcobacter is underestimated because of the limitations of currently available methods for species identification.The aim of this study was to compare the performance of five PCR based methods that target regions of 16S rRNA, 23S rRNA or gyrA genes to identify Arcobacter species, and to review previous results reported in the literature using these methods.

Results

The five tested methods were found not to be reliable. They misidentified between 16.8% and 67.4% of the studied strains; this was dependent upon the target regions of the tested

SUBMITTER: Levican A 

PROVIDER: S-EPMC3850767 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature