Detailed glycan structural characterization by electronic excitation dissociation.

ABSTRACT: The structural complexity and diversity of glycans parallel their multilateral functions in living systems. To better understand the vital roles glycans play in biological processes, it is imperative to develop analytical tools that can provide detailed glycan structural information. This was conventionally achieved by multistage tandem mass spectrometry (MS(n)) analysis using collision-induced dissociation (CID) as the fragmentation method. However, the MS(n) approach lacks the sensitivity and throughput needed to analyze complex glycan mixtures from biological sources, often available in limited quantities. We define herein the critical parameters for a recently developed fragmentation technique, electronic excitation dissociation (EED), which can yield rich structurally informative frag

SUBMITTER: Yu X 

PROVIDER: S-EPMC3851312 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature