ABC transporter activity linked to radiation resistance and molecular subtype in pediatric medulloblastoma.

ABSTRACT:

Background

Resistance to radiation treatment remains a major clinical problem for patients with brain cancer. Medulloblastoma is the most common malignant brain tumor of childhood, and occurs in the cerebellum. Though radiation treatment has been critical in increasing survival rates in recent decades, the presence of resistant cells in a substantial number of medulloblastoma patients leads to relapse and death.

Methods

Using the established medulloblastoma cell lines UW228 and Daoy, we developed a novel model system to enrich for and study radiation tolerant cells early after radiation exposure. Using fluorescence-activated cell sorting, dead cells and cells that had initiated apoptosis were removed, allowing surviving cells to be investigated before extensive proliferation

SUBMITTER: Ingram WJ 

PROVIDER: S-EPMC3851566 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature