Clinical instability of breast cancer markers is reflected in long-term in vitro estrogen deprivation studies.

ABSTRACT:

Background

Long-term estrogen deprivation models are widely employed in an in vitro setting to recapitulate the hormonal milieu of breast cancer patients treated with endocrine therapy. Despite the wealth information we have garnered from these models thus far, a comprehensive time-course analysis of the estrogen (ER), progesterone (PR), and human epidermal growth factor 2 (HER-2/neu) receptors on the gene and protein level, coupled with expression array data is currently lacking. We aimed to address this knowledge gap in order to enhance our understanding of endocrine therapy resistance in breast cancer patients.

Methods

ER positive MCF7 and BT474 breast cancer cells were grown in estrogen depleted medium for 10 months with the ER negative MDA-MB-231 cell line employed as c

SUBMITTER: Milosevic J 

PROVIDER: S-EPMC3852062 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature