A non-surgical approach for male germ cell mediated gene transmission through transgenesis.

ABSTRACT: Microinjection of foreign DNA in male pronucleus by in-vitro embryo manipulation is difficult but remains the method of choice for generating transgenic animals. Other procedures, including retroviral and embryonic stem cell mediated transgenesis are equally complicated and have limitations. Although our previously reported technique of testicular transgenesis circumvented several limitations, it involved many steps, including surgery and hemicastration, which carried risk of infection and impotency. We improved this technique further, into a two step non-surgical electroporation procedure, for making transgenic mice. In this approach, transgene was delivered inside both testes by injection and modified parameters of electroporation were used for in-vivo gene integration in germ cells. Usi

SUBMITTER: Usmani A 

PROVIDER: S-EPMC3852150 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature