Comparison of ESTs from juvenile and adult phases of the giant unicellular green alga Acetabularia acetabulum.

ABSTRACT:

Background

Acetabularia acetabulum is a giant unicellular green alga whose size and complex life cycle make it an attractive model for understanding morphogenesis and subcellular compartmentalization. The life cycle of this marine unicell is composed of several developmental phases. Juvenile and adult phases are temporally sequential but physiologically and morphologically distinct. To identify genes specific to juvenile and adult phases, we created two subtracted cDNA libraries, one adult-specific and one juvenile-specific, and analyzed 941 randomly chosen ESTs from them.

Results

Clustering analysis suggests virtually no overlap between the two libraries. Preliminary expression data also suggests that we were successful at isolating transcripts differentially expressed betw

SUBMITTER: Henry IM 

PROVIDER: S-EPMC385229 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature