Genomic sequence diversity and population structure of Saccharomyces cerevisiae assessed by RAD-seq.

ABSTRACT: The budding yeast Saccharomyces cerevisiae is important for human food production and as a model organism for biological research. The genetic diversity contained in the global population of yeast strains represents a valuable resource for a number of fields, including genetics, bioengineering, and studies of evolution and population structure. Here, we apply a multiplexed, reduced genome sequencing strategy (restriction site-associated sequencing or RAD-seq) to genotype a large collection of S. cerevisiae strains isolated from a wide range of geographical locations and environmental niches. The method permits the sequencing of the same 1% of all genomes, producing a multiple sequence alignment of 116,880 bases across 262 strains. We find diversity among these strains is principally organi

SUBMITTER: Cromie GA 

PROVIDER: S-EPMC3852379 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature