Intratumoral genetic heterogeneity in metastatic melanoma is accompanied by variation in malignant behaviors.

ABSTRACT:

Background

Intratumoral heterogeneity is a major obstacle for the treatment of cancer, as the presence of even minor populations that are insensitive to therapy can lead to disease relapse. Increased clonal diversity has been correlated with a poor prognosis for cancer patients, and we therefore examined genetic, transcriptional, and functional diversity in metastatic melanoma.

Methods

Amplicon sequencing and SNP microarrays were used to profile somatic mutations and DNA copy number changes in multiple regions from metastatic lesions. Clonal genetic and transcriptional heterogeneity was also assessed in single cell clones from early passage cell lines, which were then subjected to clonogenicity and drug sensitivity assays.

Results

MAPK pathway and tumor suppressor mut

SUBMITTER: Anaka M 

PROVIDER: S-EPMC3852494 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature