Selection of reference genes from two leafhopper species challenged by phytoplasma infection, for gene expression studies by RT-qPCR.

ABSTRACT:

Background

Phytoplasmas are phloem-limited phytopathogenic wall-less bacteria and represent a major threat to agriculture worldwide. They are transmitted in a persistent, propagative manner by phloem-sucking Hemipteran insects. For gene expression studies based on mRNA quantification by RT-qPCR, stability of housekeeping genes is crucial. The aim of this study was the identification of reference genes to study the effect of phytoplasma infection on gene expression of two leafhopper vector species. The identified reference genes will be useful tools to investigate differential gene expression of leafhopper vectors upon phytoplasma infection.

Results

The expression profiles of ribosomal 18S, actin, ATP synthase β, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and tropomyosi

SUBMITTER: Galetto L 

PROVIDER: S-EPMC3852609 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature