Visualizing chemical structure-subcellular localization relationships using fluorescent small molecules as probes of cellular transport.

ABSTRACT:

Background

To study the chemical determinants of small molecule transport inside cells, it is crucial to visualize relationships between the chemical structure of small molecules and their associated subcellular distribution patterns. For this purpose, we experimented with cells incubated with a synthetic combinatorial library of fluorescent, membrane-permeant small molecule chemical agents. With an automated high content screening instrument, the intracellular distribution patterns of these chemical agents were microscopically captured in image data sets, and analyzed off-line with machine vision and cheminformatics algorithms. Nevertheless, it remained challenging to interpret correlations linking the structure and properties of chemical agents to their subcellular localization p

SUBMITTER: Rosania GR 

PROVIDER: S-EPMC3852740 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature