Functional analysis of the rodent CK1tau mutation in the circadian clock of a marine unicellular alga.

ABSTRACT:

Background

Casein Kinase 1 (CK1) is one of few proteins known to affect cellular timekeeping across metazoans, and the naturally occurring CK1tau mutation shortens circadian period in mammals. Functional conservation of a timekeeping function for CK1 in the green lineage was recently identified in the green marine unicell Ostreococcus tauri, in spite of the absence of CK1's transcriptional targets known from other species. The short-period phenotype of CK1tau mutant in mammals depends specifically on increased CK1 activity against PERIOD proteins. To understand how CK1 acts differently upon the algal clock, we analysed the cellular and proteomic effects of CK1tau overexpression in O. tauri.

Results

Overexpression of the CK1tau in O. tauri induces period lengthening identical

SUBMITTER: van Ooijen G 

PROVIDER: S-EPMC3852742 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature