Empirical assessment of sequencing errors for high throughput pyrosequencing data.

ABSTRACT:

Background

Sequencing-by-synthesis technologies significantly improve over the Sanger method in terms of speed and cost per base. However, they still usually fail to compete in terms of read length and quality. Current high-throughput implementations of the pyrosequencing technique yield reads whose length approach those of the capillary electrophoresis method. A less obvious question is whether their quality is affected by platform-specific sequencing errors.

Results

We present an empirical study aimed at assessing the quality and characterising sequencing errors for high throughput pyrosequencing data. We have developed a procedure for extracting sequencing error data from genome assemblies and study their characteristics, in particular the length distribution of indel gap

SUBMITTER: da Fonseca PG 

PROVIDER: S-EPMC3852801 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature