The RootScope: a simple high-throughput screening system for quantitating gene expression dynamics in plant roots.

ABSTRACT:

Background

High temperature stress responses are vital for plant survival. The mechanisms that plants use to sense high temperatures are only partially understood and involve multiple sensing and signaling pathways. Here we describe the development of the RootScope, an automated microscopy system for quantitating heat shock responses in plant roots.

Results

The promoter of Hsp17.6 was used to build a Hsp17.6p:GFP transcriptional reporter that is induced by heat shock in Arabidopsis. An automated fluorescence microscopy system which enables multiple roots to be imaged in rapid succession was used to quantitate Hsp17.6p:GFP response dynamics. Hsp17.6p:GFP signal increased with temperature increases from 28°C to 37°C. At 40°C the kinetics and localization of the response are ma

SUBMITTER: Kast EJ 

PROVIDER: S-EPMC3852858 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature